Data from the manuscript: Ishii N, Suga Y, Hagiya A, et al. Dynamic simulation of an in vitro multi-enzyme system. FEBS Lett. 2007;581(3):413-420. doi:10.1016/j.febslet.2006.12.049
Footnote: Initial rates were estimated from the extent of substrate hydrolysis during 10 min incubation at 60°C. Reaction mixtures contained 10–60 mM p-NP palmitate, free and immobilised lipase (2 U),