Granulocytic nuclear lobation.

Changes in nuclear morphology during granulopoiesis are a characterising feature of human granulocytic immune system cells. The leading hypothesis for their specific function relates to cell extravasation and migration through extracellular space. Nevertheless, the mechanisms of polylobation are unclear, with the sole identified molecular determinant being lamin B-receptor. It has been long speculated that cytoskeletal forces, especially microtubule-networks, are the shaping force behind this process. Analysis of 3 different time points during 4 day granulocytic differentiation of HL60 cells. 4 biological replicates.