Studies in an early development window unveils a severe HSC defect in both murine and human Fanconi anemia [mouse]

We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between E12.5 and E14.5 developmental window. We also report for the first time that a deep HSC defect is also observed during human FA development, and that human FA FL HSCs present a transcriptome profile similar to that of mouse E12.5 Fancg-/- FL HSCs. Fancg-/- and WT LSA cells were sorted from mouse FL at E12.5 and E14.5 in three independent experiments.RNA of sorted cells was purified using RNeasy Plus Micro Kit (Qiagen) and RNA concentration and integrity evaluated with the Agilent Bioanalyzer 2100. 2 ng of total RNA is reverse transcribed following the Ovation Pico System V2 (Nugen). cDNA is then hybridized to GeneChip® MTA1.0 (Affymetrix)