RNA sequencing of medaka exposed to nZnO and Zn ion

Nano-zinc oxide (nZnO) has been widely used in various biomedical applications, but it remains unknown whether it can provide similar biological developmental requirements as Zn2+. We determined transcriptomic gene changes in medaka(Oryzias melastigma) exposed to food phases for 14 consecutive days. The treatment groups were set up as follows:70 ppm of 20 nm nZnO, 150 ppm g of 20 nm nZnO, 70 ppm Zn2+, 150 ppm Zn2+, Zn deficiency diet powder.