The Drosophila proventriculus lacks stem cells but compensates for age-related cell loss via endoreplication-mediated cell growth

Drosophila's proventriculus, a bulb-shaped organ between the foregut and midgut, aids ingestion, peritrophic membrane synthesis, and immune defense. We created a single cell transcriptional atlas of the proventriculus using single nucleus RNAseq (snRNAseq)to to identify molecular markers and use it as the foundation for for further molecular study in various proventriculus cell types. Overall design: Proventriculus from control and cachectic flies were sequenced separately, then bioinformatically pooled to make an atlas of the tissue.