Structural insights into the mechanism of rhodopsin phosphodiesterase

Crystal structures of TMD of Rhodopsin phosphodiesterase (PDB code: 7CJ3).6160 small-wedge (10°/crystal) datasets collected from loop-harvestedmicrocrystals using EIGER X 9M detector at a wavelength of 1 Å onBL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation stepwas 0.1°. The crystals belonged to space group P212121 with unit cellparameters a~66, b~74, c~117 Å. 864 datasets were indexed andintegrated using XDS with KAMO pipeline. Finally 271 integratedresults were merged at 2.6 Å resolution after CC-based clustering andoutlier rejection by KAMO in the published result.

罗丹明磷酸二酯酶（PDB代码：7CJ3）的TMD晶体结构。收集了6160个小楔形（每晶体10°）数据集，这些数据集通过EIGER X 9M探测器在1 Å波长下，在BL32XU，SPring-8上，从环状采集的微晶中获取。束斑大小约为15×10 µm²，摆动步长为0.1°。晶体属于P212121空间群，其晶胞参数为a≈66，b≈74，c≈117 Å。864个数据集使用XDS与KAMO管道进行索引和积分。最终，经过基于CC的聚类和KAMO的异常值拒绝后，将271个积分结果在2.6 Å分辨率下合并。