ChIP-Seq of Ndt80-Myc in S. cerevisiae during meiosis and sporulation
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The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae during meiosis and sporulation. Ndt80 was tagged with c-myc and the protein was immunoprecipitated with a c-myc antibody. Cells were grown in liquid YPA (2% Peptone, 2% Potassium Acetate, 1% Yeast Extract) at room temperature for 22 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000. S. cerevisiae Ndt80-myc vs. untagged control with two technical replicates for each
创建时间：
2019-05-15
