Mitochondrial genome sequences of Fireflies (Coleoptera: Lampyridae)

This dataset contains 82 published and 22 newly sequenced and assembled complete mitochondrial genomes of fireflies. From these complete mitochondrial genomes, we extracted 13 protein coding genes and 2 rRNA genes, which we aligned and curated. Finally, we built a time-calibrated phylogeny for these 104 firefly species. Description of the data and file structure We collected 22 firefly species from Europe and Central America. Bioluminiscent adults (Luciola, Photinus, Photuris and Bicellonycha) were collected using an insect net and dark adults were collected using light traps (Lamprohiza and Lampyris). High molecular weight DNA was extracted from single males using the MagAttract HMW DNA kit (Qiagen) following manufacturer’s guidelines. DNA fragment sizes and integrity were checked with a 1% agarose gel. Long DNA fragments were sequenced from a single male individual using Nanopore PromethION sequencer. We additionally collected mitochondrial genomes from GenBank for 82 firefly species.### Files and variables File: alignments.zip Description: Multiple sequence alignments of all 104 firefly species used in this study (one file per locus). We extracted 13 protein coding genes and two rRNA genes from the complete mitochondrial genomes. We combined these published genomes with our 22 mitochondrial genomes. We aligned the 13 protein coding genes using MACSE v2, using the insect mitochondrial code. We also used MACSE to replace artificial stopcodons with ‘NNN’ if these occurred within a protein coding sequence. Furthermore, we trimmed the alignments using trimAl with the option automated1. For the two ribosomal RNA genes 12S and 16S, we used the alignment software MAFFT v7 and trimAl with the option automated1. File: Lampyridae_Mito_final.tre Description: Our final estimated time-calibrated phylogeny containing the 104 firefly species. The phylogeny was estimated using RevBayes. File: mitochondrial_genomes.zip Description: Full mitochondrial genomes of the 22 newly sequenced species and 82 published species (one file per species). We assmbled the genomes using MitoFinder and MitHifi. The mitochondrial genomes were annotated using Mitoz v3.6. Code/software We analyzed the multiple sequence alignment using the software RevBayes (https://github.com/revbayes/revbayes) to estimate the time-calibrated phylogeny.