Impact of MET2 gene deletion on the growth ability of C. albicans SC5314 cells

The datasets present results on the impact of MET2 gene deletion, which encodes homoserine acetyltransferase, on the growth ability of C. albicans SC5314 cells in YNB medium supplemented with L-Met, L-Cys, or L-Ser at different concentrations.
Overnight cultures of C. albicans were washed twice with sterile phosphate-buffered saline (PBS). The cell suspensions were diluted to 2 × 10⁵ cells mL⁻¹ in YNB medium without amino acids and with ammonium sulfate. Aliquots of 100 µL were used to inoculate microtiter plate wells containing 100 µL of YNB medium (without amino acids, with ammonium sulfate) supplemented with L-Met, L-Cys, or L-Ser at varying concentrations. The microplates were incubated at 30 °C for 24 h, and cell density was measured spectrophotometrically (λ = 600 nm) using a TECAN Spark 10M microplate reader.
Medium composition:YNB medium without amino acids, with ammonium sulfate, 2% (w/v) glucose.
The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).