D-Pinitol delays renal aging by targeting the SARM1-cGAS-STING axis to improve mitochondrial function and reduce inflammation: transcriptome sequencing of HK-2 cells

This project aims to investigate the molecular mechanisms by which D-Pinitol (DP) delays renal aging through transcriptomic analysis. RNA sequencing was performed on HK-2 human renal tubular epithelial cells under three conditions: control group, D-galactose (DG)-induced aging model group, and DG + DP treatment group. Each group contained three biological replicates. Total RNA was extracted and sequenced on the Illumina platform to generate approximately 20 million clean reads per sample. Differential expression analysis, GO enrichment, and KEGG pathway analysis were conducted to identify key genes and pathways regulated by DP. Our analysis revealed that SARM1 was significantly upregulated in the aging model and markedly downregulated upon DP treatment, suggesting its critical role in DP-mediated renal protection. Further pathway enrichment indicated that DP exerts its anti-aging effects by modulating mitochondrial function and inflammation-related pathways. The raw sequencing data (FASTQ files) and processed gene expression matrices are deposited here to support the findings of our study and to enable further reanalysis by the research community.