Effect of JAKinibs and BCL6 on endothelial activation by IFNγ

The durability of interferon signaling in human aortic endothelial activation was tested. Pro-adhesive and costimulatory gene expression, phenotype, secretome and JAK/STAT phosphorylation in human primary endothelial cells were measured under chronic and transient IFNγ stimulation, with various JAK inhibitors. Primary human aortic endothelial cells, n=3 paired biological replicates per condition Ecs were untreated or treated with 30min pre-treatment with ruxolitinib (2uM), FX1, BI-3812 or BI-3802 (each at 100uM) prior to 24hr treatment with IFNg