Single cell transcriptome and surface protein expression analysis identify OX40+GITR+ pathogenic T helper 17 in anklyosing spondilytis [scRNA-seq]

We unveiled the characteristics of pTh17s, which are polyfunctional IL-17A and IFN-?-producing memory CD4+ T cells. scRNA-seq and cytometry analysis found that the co-expression of OX40 and GITR is increased in pTh17 cells. Overall design: We performed transcriptome and cytometric analysis to understand pTh17. And in vivo suppression of ligand-receptor interactions via OX40 and GITR effectively suppressed clinical arthritis and decreased pTh17 cells in the curdlan-injected SKG mouse model.