miRNAs regulated systemically by nitrogen availability in shoots and roots of Medicago truncatula

Using a dedicated split-root approach, we identified miRNAs regulated systemically by nitrogen availability in both shoots and roots of the Medicago truncatula model legume, depending on the CRA2 pathway, highlighting the phosphate-related miR399. Overall design: small RNA-seq of distant split-root or shoots of medicago truncatulla control (A17) or cra2 mutant where local split-roots were subjected to nitrogen satiety or deficitconducted in triplicate.