Supplemental Material for Mishra et al., 2020

Supplementary Figure S1 shows
the reversion analysis by the excision of P-elements
from mutant alleles of DCP2.

Supplementary Figure S2 shows
the morphological defects exhibited by escapees of adult fly trans-heterozygous
for P{GT1}DCP2BG01766/l(3)tb.

Supplementary Figure S3
shows the defects in compound eyes of the escapees having heterozygous genetic
background of the mutant l(3)tb with
lethal P-insertion allele DCP2BG01766.

Supplementary Figure S4
shows the tumorous phenotype observed in larval brain and wing imaginal discs
in trans-heterozygotes l(3)tb
/PBac{RB}DCP2e00034 and l(3)tb
/P{GT1}DCP2BG01766 similar to homozygous l(3)tb mutants.

Supplementary Figure S5
shows the results of screening of DCP2
in the l(3)tb mutants, using
overlapping primers.

Supplementary Figure S6 shows
the schematic representation of the
convergent bidirectional primer walking adopted for sequencing and alignment of
the large amplicon obtained at the candidate region in DCP2l(3)tb
homozygotes and the reads obtained on sequencing with each of the four primers.

Supplementary Figure S7 shows that the effects of ubiquitous knockdown
of Diablo does not lead to any developmental anomaly and does not
affect survival of the driven progeny.

Supplementary Table S1 depicts
the complementation status of l(3)tb
with cytologically mapped deletion lines.

Supplementary Table S2 shows the complementation analysis of l(3)tb
with lethal transposon insertion lines.

Supplementary Table S3 lists
the primers used for characterizing deletion in Df(3L)RM95.

Supplementary Table S4 shows
the overlapping set of primers for DCP2
and thermal cycler conditions of annealing temperature and extension time for
each primer pair to amplify the genomic region of DCP2 gene in the homozygous l(3)tb
mutant.

Supplementary Table S5 lists
the overlapping set of primers to amplify the genomic region in DCP2 gene for the region covered by the
DCP2_P19 set of primers in the homozygous l(3)tb
mutant.

Supplementary Table S6 lists
the overlapping set of primers and thermal cycling conditions used to amplify
the complete 5’UTR of genomic region in DCP2
gene in the homozygous l(3)tb mutant.