ChIP-seq analysis with H3K4me3 and H3K27me3 in systemic leaves of Arabidopsis thaliana locally inoculated with Pseudomonas syringae AvrRpm1

Defense activation at local pathogen challenged leaves leads to systemic acquired resistance (SAR) with defense-related genes primed for a subsequent stimulus at distal unchallenged (systemic) leaves. Our previous studies implied the possible involvements of transcription-permissive histone modification H3K4me3 and transcription-repressive H3K27me3 in systemic immunity. Our studies found that ATX1 (responsible for H3K4me3 deposition) and CLF-PRC2 (responsible for H3K27me3 deposition) both contribute to systemic defense priming and SAR. To examine changes in H3K4me3 and H3K27me3 deposition during SAR, we conducted ChIP-seq analysis in systemic leaves of Arabidopsis thaliana WT and clf-28 plants following the local inoculation of Pseudomonas syringae pv tomato AvrRpm1 or mock.