Additional file 1 of Linkage mapping and QTL analysis of flowering time using ddRAD sequencing with genotype error correction in Brassica napus

Additional file 1: Table S1. Linkage map constructed using ASMap with corrected and imputed ddRAD markers derived from an BnSOSR x BnWOSR F2 population. Table S2. Mean markers and linkage map sizes across maps generated in this study. Abbreviations: doubled haploid (DH), recombinant inbred lines (RIL), amplified fragment length polymorphisms (AFLP), Brassica 60 K genotyping array (Brassica 60 k). Table S3. Phenotypic variation in flowering time and time to bud in the F2 mapping population and the parental lines. Phenotypes of spring type BnSOSR and winter type BnWOSR parental lines are shown as means of all replicates of each type (n = 4, n = 3, respectively). Table S4. QTL identified using genome-wide single QTL scans for budding time (B) and flowering time (FT). For each QTL, the phenotypic variance explained (PVE) and additive effect (AE) are shown. Table S5. Summary of variants impacting candidate flowering time genes on chromosome C2. The QTL regions ranges from 4,345,729 to 24,916,709. Table S6. Exon coverage of candidate genes on chromosome 2 generated by whole genome skim sequencing of 16 early-flowering and 19 late-flowering F2 individuals. Table S7. Summary of intergenic variants impacting candidate genes and segregating between early-flowering and late-flowering F2 individuals. Table S8. In silico double enzyme digest analysis. Frag: Total number of fragments; FragDe: total number of different end fragments; LengthDeFrag: total length in bases of different end fragments; DeFrag100–600: total number of different end fragments between 100 bases and 600 bases; LengthDeFrag100–600: total length in bases of different end fragments between 100 bases and 600 bases; % Coverage: percentage of the reference genome covered by fragments between 100 bases and 600 bases. Table S9. Phenotype data for flowering time and budding time in the mapping population and parents. Table S10. Sample list for the 194 progeny and 7 parental individuals used in this study.