Molecular Profiling Provides Clinical Insights into Targeted and Immunotherapies as well as Prognosis for Colorectal Cancer

We performed single cell sequencing to validate the genomic-guided predictive efficacy of immunotherapy and comprehend the interactions between tumor cells and immune-system cells in cases of wtih complete response and progressive disease. Two treatment-naive colorectal cancer patients (one MSI-high/TIB-high and one POLE-mutant/TMB-UH) were recruited and received a single dose of pembrolizumab. Paired endoscopic biopsies of the tumor and adjacent normal tissue were collected for single cell sequencing at baseline and after two courses of neoadjuvant therapy.