Integration of morphological，physiological and multi-omics analysis reveals the optimal planting density improving leaf yield and active compound accumulation in Ginkgo biloba

研究了四种种植密度（对照、中密度 [MD]、中高密度 [MHD] 和高密度 [HD]）下银杏幼苗的叶片产量和质量。尽管单株叶片鲜重和干重有所降低，但在 MD-HD 下，单位面积的叶片产量连续两年增加了 60.5-138.8%。与其他密度相比，MD 导致各种生物活性次级代谢产物的积累更高，包括 13 种黄酮类化合物、银杏内酯和白叶内酯。相应地，参与黄酮类生物合成的查尔酮合酶（CHS）、查尔酮异构酶（CHI）和黄酮醇合酶（FLS）的编码基因被上调。然而，当超过MD时，生物活性次级代谢产物的含量和相关基因的表达量急剧下降。这些结果表明，作为最佳密度的 MD 不仅可以提高叶片产量，而且可以改善 G. 中活性化合物的积累。

We investigated leaf yield and quality of Ginkgo biloba seedlings under four planting densities: control, moderate density (MD), moderate-high density (MHD), and high density (HD). Although the fresh and dry weights of individual leaves decreased, the leaf yield per unit area under MD to HD increased by 60.5–138.8% over two consecutive years. Compared with other densities, MD resulted in higher accumulation of various bioactive secondary metabolites, including 13 flavonoids, ginkgolides, and bilobalide. Accordingly, the genes encoding chalcone synthase (CHS), chalcone isomerase (CHI), and flavonol synthase (FLS) involved in flavonoid biosynthesis were upregulated. However, when the planting density exceeded MD, the contents of bioactive secondary metabolites and the expression levels of the related genes decreased sharply. These results indicate that MD, as the optimal planting density, not only improves leaf yield but also enhances the accumulation of bioactive compounds in G. biloba.

搜集汇总

背景与挑战

背景概述

该数据集通过形态、生理和多组学整合分析，研究了不同种植密度对银杏叶片产量和活性化合物积累的影响。研究发现，中密度（MD）作为最佳种植密度，不仅能提高单位面积叶片产量，还能促进黄酮类等生物活性次级代谢产物的积累，相关基因表达也相应上调。