Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV) [pig liver]

We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during HEV infection in swine liver tissues. Eight-week-old specific pathogen free Large-White piglets were infected intravenously with 10^6 copies of HEV RNA or mock-infected with PBS. At the peak of excretion (8 days post-infection), liver tissues were snap-frozen in liquid nitrogen. Samples from 2 control pigs, 3 pigs infected with HEV-3c and 3 pigs infected with HEV-3f were analyzed in this study.