Repository Table R3 Phosphoproteomics IMAC n1.tab
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HeLa cells were SILAC-labeled and treated with DMSO (light) or Ro3306 (heavy, 10 µM) for 4 h. After lysis and disassembly of polysomes in low magnesium buffer, samples were mixed, and ribosomal fractions obtained by sucrose density centrifugation were subjected to Wessel-Flügge precipitation. Phosphopeptides were enriched using PhosSelect iron affinity gel IMAC beads, fractionated and analyzed by mass spectrometry followed by MaxQuant analysis.
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heiDATA创建时间：
2020-02-06
