Warm sensor protein TRPM2 is required for brain development via SP5 in hyperthermia

Next-generation sequencing (NGS) has revolutionized systems-based analysis of gene expression. The goals of this study are to compare the different transcripts between TRPM2 knockout and WT E16 cerebral brain tissue when the maternal hyperthermia was induced. Methods: Total RNA was extracted from E16 cortical tissue of TRPM2 KO and WT mice which were induced in hyperthermia at E14-E16. Then RNA was quality controlled and quantified using an Agilent 2100 Bioanalyzer. After converting to cDNA and building library, high-throughput sequencing was performed using the Illumina HiSeq 2500 platform in Annoroad Genomics. Significantly differentially expressed genes were identified when we compared Normalized Reads Count between groups with p < 0.05 and |Log2FoldChange| > 1.