Input Impedance Recordings in Neocortical Pyramidal cells

Recordings of Cellular Input Impedance in Layer V Pyramidal cells of Mouse V1 using patch-clamp recordings in brain slices. We performed intracellular recordings of visually identified pyramidal cells located in the layer V of mouse cortex using the perforated‐patch technique. To determine the input impedance at the soma, we injected sinusoidal currents in the current-clamp mode, we recorded the membrane potential response to a current input and we varied the frequencies and amplitudes.