Supplementary Data for Hua et al. (2020)

This dataset contains supplementary data for the analyses presented in:

Zhengshuang Hua, Matthew Ouellette, Andrea M. Makkay, R. Thane Papke, and Olga Zhaxybayeva. Nutrient supplementation experiments on saltern microbial communities support utilization of DNA as a source of phosphorus (under review).

Dataset contents:

Quality_Control_script.zip: In-house Perl script used for quality control processing of raw Illumina reads. See README.md file inside the archive for details.

merged_pairs.zip: Merged pairs from the rpoB and 16S rRNA data after the quality control step, removal of primers, and additional removal of low quality sequences. The sequences are in FASTA format.

rpoB_database.zip: Curated database of 627 rpoB gene sequences used for taxonomic assignment of a representative sequence of an rpoB-based OTU. Halobacteria.rpoB.627.fa file contains nucleotide sequences in FASTA format. Halobacteria.rpoB.627.taxonomy file contains the taxonomic information for each sequence.

OTU_tables_and_rep_seqs.zip: OTU tables for rpoB- and 16S rRNA-based OTUs (otu_table.rpoB.txt and otu_table.16S.txt files). Each row represents an OTU. Each column represent a sample. Values in the table show how many assembled contigs are in an OTU. Nucleotide sequences of chosen to represent each OTU are provided in rep_set_rpoB.fna and rep_set_16S.fna files, in FASTA format.