The type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis in HeLa cells and dynamic investigation upon transcription inhibition by ActD treatment

Developing a psoralen probe (PP)-based method for RNA tagging and RNA-protein complex (RP-complex) enrichment, isolating of both coding and noncoding RNAs from HeLa cells was achieved by PP enrichment. Exploring the type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis. And dynamic investigation of RNA upon transcription inhibition by ActD treatment. The type and relative distribution of RNAs in HeLa cells by PP enrichment and TRIzol, RNA readcounts in HeLa cells treating with ActD