Transformation efficiency increased in the N40 bbe02::Bbluc-aadA strain, compared to the wild-type strain N40 D10/E9.

Figshare2015-12-03 更新2026-04-29 收录

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https://figshare.com/articles/dataset/_Transformation_efficiency_increased_in_the_N40_bbe02_Bbluc_aadA_strain_compared_to_the_wild_type_strain_N40_D10_E9_/1447645

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Plasmid pBSV2G DNA is used to transform B. burgdorferi strains by electroporation. Transformation frequency is calculated by the number of gentamicin-resistant colonies per 5x108 spirochetes, from a total of two experiments.Transformation efficiency increased in the N40 bbe02::Bbluc-aadA strain, compared to the wild-type strain N40 D10/E9.

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2015-12-03