RNA-seq of CYR61-treated murine bone marrow-derived macrophages

We assess the effect of CYR61 treatment on the transcriptome of mouse bone marrow-derived macrophages. We isolated bone marrow from the tibias and femurs of C57BL/6J mice (2-5 months old). Bone marrow cells were incubated for 5-6 days in macrophage differentiation media: DMEM + 10% FBS + 10% CMG-conditioned media + 1% penicillin/streptomycin + 1% L-glutamine. After differentiation, macrophages were switched to mature macrophage media: DMEM + 10% FBS + 2% CMG-conditioned media + 1% penicillin/streptomycin + 1% L-glutamine. We treated macrophages with 0.00 or 0.50 ug/ml CYR61 (PeproTech 120-25) for 24 hrs. After incubation, we washed cells with PBS, isolated RNA, and prepared samples for RNA-sequencing.