Tandem affinity purification is the principal method for purifying and identifying stable protein complexes system-wide in whole cells. Although highly effective, this approach is laborious and imprac
The associated files are mass spec data from native nuclear extract from broccoli florets (Brassica oleracea var. italica)fractionated on an Agilent 3100 OFFGEL isoelectric focusing apparatus.
The automated processing of data generated by top down proteomics would benefit from improved scoring for protein identification and characterization of highly related protein forms (proteoforms). Her