Interaction of model phospholipid bilayer membranes with Extracellular vesicles

Extracellular Vesicles (EVs)-mediated intercellular communication is recently receiving a rising attention. Owing to their content, EVs can target cargoes to promote beneficial or pathological conditions. However, understanding the mechanisms by which EVs interact with target cells is still a challenge. We address this by a multi-technique approach, using model membranes of different compositions. We isolated EVs from N2a cells in normal conditions and treated with the oligosaccharide OligoGM1, which is known for its neurotrophic/protective functions in the frame of Parkinson’s disease. Those 2 types of EVs were characterised by Western Blotting, DLS, NTA, AFM and DSC and have been found to have different chemico-physical characteristics. We propose here the use of NR for acquiring structural information on their detailed membrane profiles and interaction with target membrane models.