Mean generation time of <i>B. subtilis</i> 1A700 vegetative cells grown under the microscope and in shake flasks in different media.

aMean generation time is given including the standard deviation. bGeneration times from individual starting vegetative cells growing into microcolonies under the microscope were calculated using SporeTracker (see Materials & Methods for details). The amount of individual starting cells analyzed and gathered from three independent biological replicates is given in brackets. cAmount of independent biological replicates is given in brackets.