Datasheet1_Transcriptional profiles of non-neuronal and immune cells in mouse trigeminal ganglia.xlsx

Non-neuronal cells constitute 90%–95% of sensory ganglia. These cells, especially glial and immune cells, play critical roles in the modulation of sensory neurons. This study aimed to identify, profile, and summarize the types of trigeminal ganglion (TG) non-neuronal cells in naïve male mice using published and our own data generated by single-cell RNA sequencing, flow cytometry, and immunohistochemistry. TG has five types of non-neuronal cells, namely, glial, fibroblasts, smooth muscle, endothelial, and immune cells. There is an agreement among publications for glial, fibroblasts, smooth muscle, and endothelial cells. Based on gene profiles, glial cells were classified as myelinated and non-myelinated Schwann cells and satellite glial cells. Mpz has dominant expression in Schwann cells, and Fabp7 is specific for SCG. Two types of Col1a2+ fibroblasts located throughout TG were distinguished. TG smooth muscle and endothelial cells in the blood vessels were detected using well-defined markers. Our study reported three types of macrophages (Mph) and four types of neutrophils (Neu) in TG. Mph were located in the neuronal bodies and nerve fibers and were sub-grouped by unique transcriptomic profiles with Ccr2, Cx3cr1, and Iba1 as markers. A comparison of databases showed that type 1 Mph is similar to choroid plexus-low (CPlo) border-associated Mph (BAMs). Type 2 Mph has the highest prediction score with CPhi BAMs, while type 3 Mph is distinct. S100a8+ Neu were located in the dura surrounding TG and were sub-grouped by clustering and expressions of Csf3r, Ly6G, Ngp, Elane, and Mpo. Integrative analysis of published datasets indicated that Neu-1, Neu-2, and Neu-3 are similar to the brain Neu-1 group, while Neu-4 has a resemblance to the monocyte-derived cells. Overall, the generated and summarized datasets on non-neuronal TG cells showed a unique composition of myeloid cell types in TG and could provide essential and fundamental information for studies on cell plasticity, interactomic networks between neurons and non-neuronal cells, and function during a variety of pain conditions in the head and neck regions.

非神经元细胞构成了90%至95%的感觉神经节。此类细胞，尤其是胶质细胞和免疫细胞，在调节感觉神经元方面发挥着至关重要的作用。本研究旨在通过单细胞RNA测序、流式细胞术和免疫组化技术产生的已发表数据和自身数据，对未接触过（naïve）雄性小鼠的三叉神经节（TG）非神经元细胞进行鉴定、特征描述和总结。三叉神经节包含五种类型的非神经元细胞，即胶质细胞、成纤维细胞、平滑肌细胞、内皮细胞和免疫细胞。在胶质细胞、成纤维细胞、平滑肌细胞和内皮细胞方面，出版物之间达成了共识。基于基因特征，胶质细胞被划分为有髓和无髓的施万细胞以及卫星胶质细胞。Mpz在施万细胞中具有主导表达，而Fabp7是SCG的特异性标记。在TG中，两种类型的Col1a2+成纤维细胞被区分开来。通过定义明确的标记物，检测到了血管中的TG平滑肌细胞和内皮细胞。本研究报道了TG中三种类型的巨噬细胞（Mph）和四种类型的嗜中性粒细胞（Neu）。Mph位于神经元体和神经纤维中，并通过Ccr2、Cx3cr1和Iba1作为标记物的独特的转录组特征进行亚分组。数据库的比较显示，1型Mph与脉络丛低（CPlo）边界相关巨噬细胞（BAMs）相似。2型Mph与CPhi BAMs具有最高的预测分数，而3型Mph则具有独特的特征。S100a8+嗜中性粒细胞位于TG周围的硬脑膜中，并通过聚类和Csf3r、Ly6G、Ngp、Elane和Mpo的表达进行亚分组。已发表数据集的整合分析表明，Neu-1、Neu-2和Neu-3与大脑Neu-1组相似，而Neu-4则与单核细胞衍生的细胞相似。总体而言，所生成和总结的非神经元TG细胞数据集展示了TG中髓系细胞类型的独特组成，并能为细胞可塑性研究、神经元与非神经元细胞之间的互作网络以及头部和颈部区域各种疼痛条件下的功能研究提供关键和基础信息。