Long-read nanopore sequencing (Nanopore-seq) of PQBP1 knockdown in ovarian cancer cells

To investigate the comprehensive function of PQBP1 in the regulation of RNA splicing, we established Dox-inducible PQBP1 knockdown HEY cell line. We then performed transcript expression profiling analysis using data obtained from long-read nanopore-seq of PQBP1 knockdown and control HEY cells (three biological replications of each sample). Overall design: Comparative transcript expression profiling analysis of Nanopore-seq data for Dox-inducible PQBP1 knockdown HEY cells (shNC, shPQBP1). The assay was performed on three biological triplicates.