Prunus persica Genome sequencing and assembly

To reveal the transcriptomic responses of peach fruit to BABA elicitation and R. stolonifer infection, RNA of the peaches in the three treatments (control, BABA, inoculation, BABA + inoculation) were extracted following subsequent mRNA enrichment with oligo (dT)-rich magnetic beads. The enriched mRNA was then fragmented into small pieces before the first- and second-strand cDNA synthesis. Double-strand cDNAs were then purified, end repaired, polyadenylated, and further ligated to Illumina sequencing adapters. The adapter-ligated fragments were selected by PCR amplification, subjected to agarose gel electrophoresis, and sequenced using an Illumina HiSeqTM 2500 instrument.