Genome-wide analysis of KAP1 recruitment in mouse regulatory T cells

We aim to determine the role of KAP1 in regulatory T cells. KAP1 is originally defined as a chromatin remodeler but there are some evidence suggesting KAP1 also works as a transcription activator. Because Treg specific KAP1 deficient mice spontaneously develop autoimmune disease, KAP1 seems to be an important transcription factor for Tregs. To address the genes which are regulated by KAP1, we performed ChIP-seq of KAP1 using mouse Tregs. We found large part of KAP1 was rectuited to transcription start sites of T cell associated genes. Regulatory T cells were sorted and cultured for two weeks. Expanded Tregs were subjected and nuclear lysate was immunoprecipitated with anti-KAP1 antibody. Precipitated chromatin was sequenced by Hiseq2500.