Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in C. elegans (fluorescence microscopy data)

This project has been submitted by the Maria Doitsidou Lab.<br> <br> Project contents:<br> This project contains datasets of z-stack images of <em>C. elegans</em> strains used to study how the gut microbiome affects Parkinson’s disease. Each strain contains a chromosomal insertion containing YFP fused to α-synuclein (pkIs2386[Punc-54::α-synuclein::YFP + unc-119(+)]). The following<em> C. elegans</em> strains were used and/or created for this project:<br> NL5901 pkIs2386[Punc-54::α-synuclein::YFP + unc-119(+)]<br> MDH586 daf-2(e1370) III; pkIs2386<br> MDH585 daf-16(mu86) I; pkIs2386<br> MDH587 hsf-1(sy441) I; pkIs2386<br> MDH657 daf-2(e1370) III; daf-16(mu86) I; pkIs2386<br> MDH614 daf-2(gk390525) III; pkIs2386<br> MDH611 eat-2(ad465) II; pkIs2386<br> MDH711 lagr-1(gk331) I, pkIs2386<br> MDH725 sptl-3(ok1927) II; pkIs2386<br> MDH724 asm-3(ok1744) IV; pkIs2386.<br> <br> High magnification (40x objective) z stack images of the head region were obtained by using a Zeiss Axio imager 2 microscope.<br> <br> <br> Aim:<br> Study how a probiotic<em> B. subtilis</em> strain affects alpha-synuclein protein aggregation.<br> <br> Main results:<br> The authors showed that the probiotic<em> B. subtilis</em> strain PXN21 inhibits and clears a-synuclein aggregation in a <em>C. elegans </em>model. The bacterium acts via metabolites and biofilm formation to activate protective pathways in the host, including DAF-16/FOXO and sphingolipid metabolism.<br> <br> Contributors:<br> Maria Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andres Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall and Maria Doitsidou<br> <br> These datasets were used in the following publication:<br> <br> Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in <em>C. elegans</em><br> <br> Maria Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andres Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall and Maria Doitsidou<br> <br> Cell Reports January 14, 2020 30 367-380; first published January 14, 2020 https://doi.org/10.1016/j.celrep.2019.12.078