Total RNA-Seq in TRAF4-overexpressing castration-resistant xenograft tumors and the control castration-responsive tumors

We reported the tumor total gene expression TRAF4-overexpressing castration-resistant xenograft tumors and the control castration-responsive tumors TRAF4 overexpressing or control LNCaP cells were injected into NOD/SCID mice subcutaneously. When tumors reach approximately 1cm in diameter, mice were were anesthetized using isoflourane and surgically castrated using standard surgical technique. For in vivo studies, 5-to 6-week-old male SCID mice (The Jackson Laboratory) were used for experimental castrate resistant prostate cancer study.