Supplementary Information S1 - Novel Mitochondrial Substrates of Omi Indicate a New Regulatory Role in Neurodegenerative Disorders

All spots were corrected for background using the integrated density (ImageJ software). In Table S1 and S2 LDH and Spot #4, of the (-) ucf-101 and OMI.S306C/G399S gels respectively, were < background, denoted here as “neg”. Control spots were chosen arbitrarily to compare overall loading densities. In all cases the FLAG.OMI.S306C control spots were less concentrated than those in OMI double mutant samples, implying the intensity of the identified spots was under-estimated by approximate factor of 2 to 2.5X. The (-) ucf-101 control spots were slightly greater or slightly less concentrated than their equivalent in the (+) ucf-101.
(0.02 MB DOC)