Table_1_A Genome-Wide Association Study Identifies New Loci Involved in Wound-Induced Lateral Root Formation in Arabidopsis thaliana.XLSX

Root systems can display variable architectures that contribute to nutrient foraging or to increase the tolerance of abiotic stress conditions. Root tip excision promotes the developmental progression of previously specified lateral root (LR) founder cells, which allows to easily measuring the branching capacity of a given root as regards its genotype and/or growth conditions. Here, we describe the natural variation among 120 Arabidopsis thaliana accessions in root system architecture (RSA) after root tip excision. Wound-induced changes in RSA were associated with 19 genomic loci using genome-wide association mapping. Three candidate loci associated with wound-induced LR formation were investigated. Sequence variation in the hypothetical protein encoded by the At4g01090 gene affected wound-induced LR development and its loss-of-function mutants displayed a reduced number of LRs after root tip excision. Changes in a histidine phosphotransfer protein putatively involved in cytokinin signaling were significantly associated with LR number variation after root tip excision. Our results provide a better understanding of some of the genetic components involved in LR capacity variation among accessions.

根系统可呈现多样化的结构，这些结构有助于养分觅食或增强对非生物胁迫条件的耐受性。通过根尖切除操作，可促进先前指定的侧根（LR）创始细胞的发展进程，从而便于测量特定根系的分支能力，这一能力与其基因型及/或生长条件密切相关。在本研究中，我们描述了在根尖切除后，120个拟南芥（Arabidopsis thaliana）种质在根系结构（RSA）方面的自然变异。利用全基因组关联分析，将RSA受损伤诱导的变化与19个基因组位点相关联。针对与损伤诱导的LR形成相关的三个候选位点进行了研究。At4g01090基因编码的假想蛋白中的序列变异影响了损伤诱导的LR发育，其功能缺失突变体在根尖切除后表现出LR数量的减少。参与细胞分裂素信号传导的组氨酸磷酸转移蛋白的变化与根尖切除后LR数量变异显著相关。我们的研究结果加深了对某些在种质间侧根能力变异中起作用的遗传成分的理解。