Gene expression profile at single cell level of goblet cells (GCs) from the distal colon (DC) and the 8th portion of the small intestine (Si8)

Objective: Benign prostatic hyperplasia (BPH) is a common disease in men over 50 years old, which seriously reduces the quality of life of patients. At present, the transcriptome changes of gene expression profile in benign prostatic hyperplasia have not been explored to a great extent. Methods: In this study, single-cell RNA sequencing was performed on the isolated prostate tissue of BPH mice, and its cell types were identified and enriched. In addition, we constructed the cell communication network and sequence analysis, revealing its receptor-ligand pair and its transmission signals with other cells. Results: The results revealed the changes in 11 cell subpopulations including B cells, DC, Endothelial cells, Epithelial cells, Fibroblast & Myofibroblast, Macrophage, Mast cells, Satellite cells, Schwann cells, Smooth Muscle cells, and T Cell & NK & NKT in the microenvironment at various stages of the occurrence and development of benign prostatic hyperplasia, and updated the understanding of Epithelial cells, Fibroblast & Myofibroblast cell markers. Conclusion: Our study promotes the understanding of cellular dynamic changes and potential molecular mechanisms in the development of BPH. ICR mice (5 weeks old, male) were housed in specific pathogen-free conditions with free access to water and mice chow. The mice were anesthetized by intraperitoneal injection of pentobarbital sodium, and their bilateral testes were removed by aseptic operation through the scrotum. After 1 week of natural recovery, the mice were subcutaneously injected with 7.5 ml/kg of testosterone propionate every day. The control group was injected with 200 μl soybean oil every day. After 3 weeks, the mice were anesthetized and bled to death. The tissues around the prostate were stripped and single-cell sequencing was performed.