Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability II. Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability II

Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, and as a translational repressor, but their functions in cells are incompletely understood. We have analyzed these questions by a combination of ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs. Overall design: It includes total 14 samples. pat1, pat1dhh1 (triplicates) and edc3scd6 (duplicates) were subjected to RNA-Seq. Separately, WT and dcp2 in triplicates (6 samples) were subjected to Rpb1 ChIP-seq. All strains were grown in YPD at 30ºC till the OD600 ~0.6