MALDI Imaging Mass Spectrometry of a Mouse Kidney

Instrument: Autoflex III Smartbeam (Bruker, Bremen, Germany) Linear mode (m/z 2000 - 20000) Negative ion mode Matrix: 2,4,6-Trihydroxyacetophenone Spatial resolution: 150 µm Tissue - Mouse Kidney (BI/6, C57BL/6JRccHsd) - Healthy - Snap frozen in liquid N2 immediately after harvest - 12 µm section (on Leica cryostat @ - 30°C) Matrix application Sublimation @ 160°C for 12 min 30 sec Recrystallization With 1 mL of 5% MeOH, 5% Acetic Acid @ 80°C for 2 min 30 sec (starting from RT) Tissue wash According to Yang and Caprioli, Anal. Chem. 2011, 83, 5728-5734 ; Rinse protocol D