Changes in APE1 conformation in presence of lucanthone and hycanthone.

Differences in CD conformation in presence of Lucanthone and Hycanthone. Data in Table 1 represent the CD analysis of APE1 in presence of lucanthone and hycanthone (Figure 7) which are shown as changes in α helix, β sheet and unordered conformation of APE1 protein. APE1 protein (5 mg/ml), 50 µl (250 µg) in APE1 buffer (50 mM HEPES, 150 mM KCl, 5 mM MgCl2), was mixed with lucanthone/hycanthone (1 mg/ml), 50 µl (50 µg), incubated at 37°C for 60 min and far UV-CD spectra were recorded at NSLS U11 beam line at BNL. These data are representation of three independent repeats.