Transcriptional profiling of mammalian preimplantation embryos

We developed an adapted version of Smart-seq2, which we call Smart-seq+5', to capture both internal transcript fragments, as well as 5' transcript information, in mammalian preimplantation stages on a single-embryo level. Focusing specifically on transposable elements, we find many conserved patterns and features shared between mouse, pig, cow, rabbit and rhesus. Transcriptional profiling using an adapted version of the Smart-seq2 protocol during preimplantation stages of mouse, pig, cow, rabbit and rhesus