Transcriptomic analyses of improved cold tolerance in cucumber (Cucumis sativus L.) caused by Exogenous chitosan oligosaccharide

Purpose: the goals of this study are to provide a theoretical basis for the use of chitosan oligosaccharide to alleviate the damage caused by cold stress in cucumber growth and development. Overall design: The transcriptome sequence were generated by deep sequencing with three repeats using Illumina. The sequence reads that passed filters were mapped tocucumber genome (http://cucurbitgenomics.org/organism/18) using HISAT2 software. The differently expressed genes were identify by |log2(FoldChange)| > 0 & p < 0.05, and qRT–PCR validation was performed using SYBR Green assays