RNA-Seq gene profiling of retinal ganglion cells in Math5Cre-driven Knockout of Ezh2 in mice at P0

Epigenetic mark deposition during embryonic development contribute to postnatal homeostasis and tissue stability. Previously, we found out that Ezh2 contributes critically to the function and postnatal cell survival in bipolar cells in the retina. (Yan et al. Postnatal onset of retinal degeneration by loss of embryonic Ezh2 repression of Six1. Sci Report. doi:10.1038/srep33887) In this study, we used RNA-seq to define up- and down regulated genes in Math5CreEzh2f/f mice which express a selective deletion of Ezh2 in retinal ganglion cells (RGC). Gene expression was compared to wild type murine retinal cells. mice pups (Math5Cre+;Ezh2f/f or wild type) were sacrificed at P0 and the retina was harvested immediately. After enzymatic digestion, retinal ganglion cells (RGC) were purified from other retinal cells using magnetic labelling with anti-CD90-antibody which binds specifically to RGC. After elution, samples were immediately proceed to RNA extraction. We collected triplicates for each cell samples. Library was prepared with NEBNext Poly A, Sequencing was done with Mid-Output single read 75 cycles flow cell. Lucy, J, Wong