Dysbiosis monitoring in healthy adults and patients with aggressive non-Hodgkin lymphoma during chemoimmunotherapy

The aim of this study was to longitudinally monitor the microbial composition of patients with aggressive non-Hodgkin lymphoma disease undergoing chemoimmunotherapy. Microbial diversity metrics obtained from 16S rRNA gene sequencing were compared to flow-cytometric microbial profiling. Fluoresbrite BB Carboxylate microspheres 1 and 0.5 µm (Polyscience, USA) were used to align the instrumental settings. Calibration of optics and laser performance with eight peak Rainbow Calibration Particles (Spherotech Inc., USA) was performed at regular intervals during a measurement day.