axiom 820K array Triticum aestivum. Triticum aestivum

The introgression of novel genes from progenitors and related species hasbeen widely employed to improve the agronomic characteristics of hexaploid wheat. Thisapproach, however, has been hampered by the lack of markers that can be used to trackintroduced chromosome segments. Here, we describe the identification of a large number ofsingle nucleotide polymorphism-based markers that can be used to genotype hexaploid wheatand to identify and track introgression from a variety of sources representing its secondary andtertiary gene pools. We have validated our markers using a high density Axiom® genotypingarray. Use of the ultra-high density platform to genotype a range of diploid, tetraploid andhexaploid wheat lines and wheat relatives has confirmed the utility of the markers across arange of species.