The impact of eosinophils on the in vitro differentiation of osteoclasts

To investigate the role of eosinophils on osteoclast differentiation from bone marrow-derived monocytes (BMMs), BMMs were cultivated in the presence or absence of eosinophils and eosinophil supernatant as compared with unstimulated control. Overall design: The mRNA expressional changes in osteoclasts upon stimulation with eosinophils or eosinophil supernatant were anlaysed by bulk RNA sequencing