Table 1 in Metabolism of ganoderic acids by a Ganoderma lucidum cytochrome P 450 and the 3 - keto sterol reductase ERG 27 from yeast

Table 1 Strains used in this study. StrainDescriptionSource/Ref.E. coli strainsBL21(DE3)general expression hostInvitrogenZY28ABL21 harboring empty plasmid pET28AThis studyZYERG27BL21 harboring plasmid pET28A-ERG27This studyS. cerevisiae strainsCEN.PK113-3Cgeneral expression hostEUROSCARFBY4742MAT α; his 3Δ1; leu 2Δ0; lys 2Δ0; ura 3Δ0EUROSCARFZY00CEN.PK113-3C harboring plasmid pESC-dTrpThis studyZYN00CEN.PK113-3C harboring plasmid pESC-dTrp-This studyNCP1ZYN02CEN.PK113-3C harboring plasmid pESC-dTrp-This studyNCP1-CYP512U6GLCPR113-3CCEN.PK113-3C deletion NCP1 with GLCPRThis studyZYGL00GLCPR113-3C harboring plasmid pESC-dTrp-This studyGLCPRZYGL02GLCPR113-3C harboring plasmid pESC-dTrp-This studyGLCPR-CYP512U6