Transcriptional profiling of the mid-proliferative phase eutopic endometrial samples from women with endometriosis

Purpose- To identify the pathways and processes that are dysregulated in the eutopic endometrium of women with endometriosis Methods- RNA sequencing was used to detect and quantify the transcripts encoded by the whole genome in the eutopic endometrium. Mid-proliferative phase eutopic endometrial samples from women with (n=4) and without endometriosis (n=3) were processed for RNA sequencing and the data were compared to identify the transcripts displaying differential abundance in women with endometriosis, compared to those without endometriosis (controls) Overall design: Total RNA isolated from mid-proliferative phase eutopic endometrial samples from women with (n=4) and without endometriosis (n=3) were enriched for messenger RNA (mRNA) using poly (A) selection. Strand-specific libraries were synthesised, adapter ligated, amplified and sequenced to obtain paired-end reads using Illumina Next Seq 500 platform. The data were analysed using the Tuxedo pipeline to detect differentially abundant transcripts in the endometrial samples from women with endometriosis, compared to those without endometriosis. This was followed by annotation for the pathways and processes enriched by the differentially expressed transcripts in endometrial samples from women with endometriosis.