Mode of action of anticancer sulfonamides

HCT116 or K562 cells treated with either DMSO or E7820 were analyzed with data independent acquisition (DIA).
The protein alkylation and digestion procedure followed the Filter Aided Sample Preparation (FASP) method, with some modification. Tryptic peptides from the whole cell lysate or immunoprecipitation samples were analyzed in a nano-flow LC-MS/MS system using a Q Exactive™ HF mass spectrometer (Thermo Fisher Scientific) coupled with an online UltiMate(R) 3000 Rapid Separation LC (RSLC, Dionex) and an HTC PAL sample injector (CTC Analytics, Zwingen, Switzerland) fitted with a microcapillary column (360 nm outer diameter (OD) × 100 μm ID), which was packed with < 20 cm of ReproSil C18-AQ 5 μm beads (Dr. Maisch GmbH) and equipped with an integrated electrospray emitter tip (P-2000 laser-based puller; Sutter Instruments).